What アニメ moment did あなた find saddest?

(For me, I have several ones: When Ace is killed によって Akainu in One Piece and Luffy cries his little ハート, 心 out, then loses his mind from despair; when Spike had finally met up with Julia and then she is killed によって the Syndicate in Cowboy Bebop, as well as Spike then dying a few episodes later; when Otonashi and Kanade are separated, right at the end of エンジェル Beats... Kanade disappears, but Otonashi stays; when ジン Ichimaru was killed によって Sosuke Aizen in Bleach, then Rangiku finds his dying body and is cut up about it; when Coyote Starrk, an Espada in Bleach, is killed によって his opponent, even though didn't really want to fight... He had to follow orders; lastly, near the end of K-On when Azunyan realises that Yui, Mio, Ricchan and Mugi will be at college and she'll be alone.)